TubeSpin® Bioreactor

TPP TubeSpin® Bioreactors have proven their advantages in large scale screening and optimizing of suspension cell system process development. The important parameters for the production upscaling can be evaluated with small amounts maximizing the number of parallel experiments at the same time. This lowers the costs and increases the efficiency of the process.

International design patent: The innovative design of the TubeSpin® Bioreactor 87600 was registered with the World Intellectual Property Organization (OMPI) in 2023!

Features of the TubeSpin® Bioreactors

TubeSpin® Bioreactor 15: working volume 1–15 ml
- conical or round bottom
- filter cap with 5 different openings (A, B, C, D, E)
TubeSpin® Bioreactor 50: working volume 1–35 ml
- conical or round bottom
- filter cap with 5 different openings (A, B, C, D, E)
TubeSpin® Bioreactor 450: working volume up to approx. 300 ml
- conical bottom
- filter cap with 10 equal openings
- additional replacement cap available: “solid” #87355, “filter” #87356
TubeSpin® Bioreactor 600: working volume up to approx. 400 ml
- conical bottom
- filter cap with 10 equal openings
- additional replacement cap available: “solid” #87355, “filter” #87356
- International design patent: The innovative design of the TubeSpin® Bioreactor 87600 was registered with the World Intellectual Property Organization (OMPI) in 2023

Filter caps for optimal gas exchange
Fit standard centrifuge apparatus
Spin: 15'500 x g or 3'500 x g
Matching adapter plates for shakers for cultivation of suspension cells available

Racks and screw caps for bioreactors:

Replacement screw caps for large bioreactors
Rack for bioreactor 450/600 ml
Rack for bioreactor 15/50 ml
TPP adapter trays for shakers from Kuhner or Infors available

Recommendations for spin, throw and shaking speed can be downloaded here.

Good to know!

Recommendation

Orbital throw and rotational speed are important factors for the success. Pay also attention to the maximal weight with which the shaker can be loaded.

From swirling of wine to bioreactors - the essence of orbital shaking

An interesting film from scientists at EPFL that have managed to model the shape of the wave created in a glass of wine while swirling it. They figured out that given parameters allow reproducing the exact wave form, whatever the size of the recipient. These finding are useful to better calibrate orbital shaking bioreactors for cell culture. See original scientific footage (source: youTube).

Water loss

If small culture volumes are incubated over a longer period of time, the loss of water influences the growth of cells.
By the closure of one or several openings above the gas permeable and sterile PTFE membrane, this water loss can be minimized.

Steril gas exchange is guaranteed through the 0.22 µm pore size PTFE filter membrane.

Downloads

Instruction for use, IFU
TechDoc Design Patent 87600
Air throughput of screw caps
Air flow rate in screw caps
Racks
Resistance to chemicals
Optimal filling volume
Kuhner Shaker Accessories
Shaker recommendations and FAQ bioreactor 15/50 mL
Orbit-rpm calculations
Are TPP products medical devices?

Shaker Information

Kuhner Shaker
Infors Multitron / Minitron

Information on Centrifuges for Bioreactors

This information has been verified by the manufacturers of the centrifuges and provided to TPP. TPP assumes no liability for the correctness and completeness of the data. If you have any questions, please be sure to contact the technical service of the respective manufacturers or your local distributor

Sigma Centrifuges for 87450/87600
Sigma brochure 8KS
Hermle Centrifuge info
Hettich Centrifuge info 87450
ThermoScientific Centrifuge info 87450 / 87600
Eppendorf Centrifuge info

Type	Product No.	Version	Volume (ml)	Dimensions (mm)	Material	Max g Force	Qty / Bag	Qty / Case	Case (kg)
	87015	15 ml, conical, filter cap	15	17.1 x 120	PP	15500	40	800	7.1
	87017	15 ml, round, filter cap	15	17.1 x 120	PP	15500	40	800	7.1
	87050	50 ml, conical, filter cap	50	30 x 115	PP	15500	20	180	3.1
	87056	50 ml, round, filter cap	50	30 x 115	PP	15000	20	180	3.1
	87450	450 ml, conical, filter cap	450	96 x 145	PP	3500	1	32	3.0
	87600	600 ml, conical, filter cap	600	98 x 183	PP	3500	1	26	3.5
	99013	Rack for 87600	3 x 87600	366 x 146 x 70	PP		1	6	2.4
	99017	Rack for 15 / 50 ml tube	18 x 15 ml / 10 x 50 ml	209 x 98 x 62	PP		1	45	5.1
	87355	screw cap "solid"	450/600	70 x 21	PE	--	1	36
	87356	screw cap "filter"	450/600	70 x 21	PE	--	1	36
	---	TPP Shaker Tray	---	---	---		---	---
	87414	Kuhner tray 162/50	162 x 50 ml	800 x 420	Alu		1	1	12
	87411	Kuhner tray 32/450	32 x 450 ml	800 x 420	Alu		1	1	12
	87412	Kuhner tray 20/450	20 x 450 ml	500 x 420	Alu		1	1	10
	87413	Kuhner tray 16/450	16 x 450 ml	420 x 420	Alu		1	1	10
	87431	Infors Multitron tray 32/450	32 x 450 ml	850 x 470	Alu		1	1	12
	87433	Infors Minitron tray 16/450	16 x 450 ml	480 x 420	Alu		1	1	10
	87611	Kuhner tray 32/600	32 x 600 ml	800 x 420	Alu		1	1	10.2
	87612	Kuhner tray 20/600	20 x 600 ml	500 x 420	Alu		1	1	7.0
	87613	Kuhner tray 16/600	16 x 600 ml	420 x 420	Alu		1	1	6.5
	87631	Infors Multitron tray 32/600	32 x 600 ml	850 x 470	Alu		1	1	10.7
	87633	Infors Minitron tray 16/600	16 x 600 ml	480 x 420	Alu		1	1	7.0

Opening

87015 + 87017: 5 x openings: A = 0.4 mm, B = 0.6 mm, C = 1.0 mm, D = 1.25 mm, E = 1.5 mm

87050/87056: 5 x openings: A = 0.4 mm, B = 0.6 mm, C = 1.0 mm, D = 1.5 mm, E = 2.0 mm

87450/87600: 10 x openings with size 4.0 mm each